Fig. 6.
Fig. 6. Biochemical and functional effects of Tpo on erythroid cell line G1E-ER2. / (A) G1E-ER2 cells were starved for 5 hours and cultured for 48 hours in the presence of 50 ng/mL SCF or TPO or 2 U/mL Epo. Apoptosis was measured by staining the cells with Annexin and analyzed by flow cytometry as described in “Materials and methods.” (B) G1E-ER2 cells were starved for 6 hours and stimulated with TPO for indicated time or left unstimulated. Immunoprecipitation (IP) was performed by using an anti–c-mpl antibody, followed by Western blot (WB) analysis using an antiphosphotyrosine antibody. Top panel shows the phosphorylation of c-Mpl, and the bottom panel shows the expression of total c-Mpl protein in each lane. Data represent the mean values of triplicate samples from 4 experiments; error bars represent SEM.

Biochemical and functional effects of Tpo on erythroid cell line G1E-ER2.

(A) G1E-ER2 cells were starved for 5 hours and cultured for 48 hours in the presence of 50 ng/mL SCF or TPO or 2 U/mL Epo. Apoptosis was measured by staining the cells with Annexin and analyzed by flow cytometry as described in “Materials and methods.” (B) G1E-ER2 cells were starved for 6 hours and stimulated with TPO for indicated time or left unstimulated. Immunoprecipitation (IP) was performed by using an anti–c-mpl antibody, followed by Western blot (WB) analysis using an antiphosphotyrosine antibody. Top panel shows the phosphorylation of c-Mpl, and the bottom panel shows the expression of total c-Mpl protein in each lane. Data represent the mean values of triplicate samples from 4 experiments; error bars represent SEM.

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