A narrow region within the D2 domain of VWFpp is important for VWFpp/VWF association and storage.

(A) Schematic representation of chimeric human/canine VWFpp and summary of storage and multimerization data. The domain structure of VWFpp is depicted at the top and chimeric VWFpp's are depicted below. Portions of VWFpp containing human sequence are depicted in black and those containing canine sequence in white. The summarized data are for coexpression of the chimeric VWFpp in trans with human Δpro. The area of overlapping human sequence for those chimeric VWFpp's that sorted human Δpro to storage is shown between the 2 vertical lines; it lies between Glu386 and Lys477 in the D2 domain. (B) Sequence comparison of human and canine VWFpp beginning at the D2 domain. The human sequence is listed on the top line, with differences in canine sequence listed below; conserved amino acids are depicted with periods.