Dose dependence of the effect of G-CSF on colony formation.

Bone marrow cells (2.5 × 10⁴) of normal mice, Tg mice expressing wild-type G-CSFR, and mutant receptors with a 730 or 717 amino acid truncation (WT⁺, 730⁺, and 717⁺, respectively), and their littermates (WT⁻, 730⁻, and 717⁻, respectively) were incubated in methylcellulose culture with varying concentrations of G-CSF. Dotted lines represent littermates of the Tg mice.