Phenotype of 5T2MM cells before injection.

Bone marrow cells isolated from tumor-bearing 5T2MM cells were phenotyped by flow cytometry. 5T2MM myeloma cells were selected by staining with anti-5T2MM–specific antibodies as described in Figure1A. Expression of CD45, CD138, and IL-6Rα on gated 5T2MM is shown. Results are expressed as bivariate plots. x-axis: expression level of the indicated antigen; y-axis: autofluorescence; no marker was used in this channel (FL-4).