Fig. 4.
Epinephrine-stimulated cAMP production and adhesion to laminin exhibit similar time courses.
(A) SS RBCs from 2 different patients were treated with 1 × 10−2 μM epinephrine and lysed after 1, 5, 15, or 30 minutes as indicated. Total cAMP levels were measured in 1 × 108 SS RBCs/condition by enzyme-immunoassay (“Materials and methods”). (B) Adhesion of SS RBCs from 2 different patients to immobilized laminin was measured using the flow adhesion assay after treatment for 1, 5, 15, and 30 minutes with 1 × 10−2 μM epinephrine. Baseline adhesion is set at 1, and fold change from baseline adhesion at each time point is shown (lines connecting the individual time points do not represent the best fit line, but serve as a visual aid).
