PARP cleavage in CLL cells.

(A) Generation of p85 PARP in CLL cells preincubated for 1 hour with no addition or with 80 μM chlorambucil and subsequently incubated for 12 hours with the indicated supplements. P indicates plasma; HA, human albumin; SER, human serum; dSER, delipidated human serum; FT, Cibacron blue flow-through. (B) Densitometric quantitation of PARP cleavage data of panel A. Blots were probed with an actin antibody as a control for loading. Data are representative of 4 experiments.