Fig. 9.
Uptake of FITC-rHA by CLL cells.
(A) Confocal micrograph of a CLL cell incubated with FITC-rHA for 30 minutes prior to fixation. Original magnification × 2500. (B-D) FACScan analysis of CLL cells exposed to FITC-rHA. (B) Effect of 30-minute preincubation with 1.5 mM NaN3 on uptake of FITC-rHA. (C-D) Competitive effect of unlabeled rHA on the uptake of FITC-rHA. Concentrations of added unlabeled rHA are indicated in individual panels. NC indicates no competing unlabeled rHA. The dotted lines (B-D) denote the fluorescence of CLL cells incubated with FITC-rHA at 4°C. All other incubations were at 37°C.
