HTSU-IgG binds to resting and activated primary cells of the immune system.

CD4⁺ (A-B) and CD8⁺ T cells (C-D), B cells (E-F), monocytes (G), monocyte-derived macrophages (H), and NK cells (I-J) were isolated from adult peripheral blood leukopaks as described in “Materials and methods.” The cells were either tested immediately for HTSU-IgG binding (80 ng/mL) (left panels) or activated for 7 days and reassayed for HTSU-IgG binding (right panels). Binding to the SUA-IgG (200 ng/mL) was no more than 0.8% of the HTSU-IgG binding for these samples. Data are from a representative experiment out of 3 performed. HTSU-IgG binding is on the y-axis and FL-2 is on the x-axis.