HTSU-IgG blocks proliferation in a mixed lymphocyte reaction.
PBMCs from 2 healthy adult donors were isolated. PBMCs from one donor served as responder, and the PBMCs from another donor were irradiated and used as stimulator cells as described in “Materials and methods.” The stimulator cells were incubated with media containing various dilutions of HTSU-IgG or with SUA-IgG, media alone, or with anti-CD2 antibody (10 μg/mL; positive control) for 30 minutes. Responder cells were then added, the cultures incubated for 6 days, and the amount of proliferation determined from the amount of3H thymidine incorporated during a 20-hour pulse, as described in “Materials and methods.” The percent proliferation was determined using the following formula: (cpm of experimental/cpm of media-only control ×100). Each bar represents the average of triplicates. Data are a representative experiment of 3 performed.
