Transferrin receptor (TfR) and UbcH5A mRNA expression normalized to actin mRNA levels in liver biopsy samples from 7 control subjects and 7 patients with untreated

HFE-associated hemochromatosis. Panel A shows expression of transferrin receptor (TfR); panel B shows expression of UbcH5A mRNA. For expression analyses, a 2-step, real-time RT-PCR on the LightCycler system was used. TfR/actin and UbcH5A/actin ratios were calculated using LightCycler Relative Quantification Software Version 1.0 (Roche Molecular Biochemicals), which provides a fully automated, efficiency-corrected, relative quantification. Mean values are shown. The error bars represent 95% confidence intervals. The differences between TfR/actin and UbcH5A/actin ratios are statistically significant (P < .01 for TfR/actin ratio; P < .05 for UbcH5A/actin ratio).