Fig. 6.
Fig. 6. A low concentration of collagen-induced, αIIbβ3-mediated aggregation drives ADP secretion and TxA2 production. / Wild-type platelets were incubated with 1B5, a hamster monoclonal antimouse αIIbβ3 antibody (10 μg/mL), or control hamster IgG (10 μg/mL) for 10 minutes before stimulation with a low concentration (2.5 μg/mL) of collagen. 1B5 inhibits fibrinogen binding to αIIbβ3, thereby preventing aggregation (A). Inhibition of aggregation substantially diminished ATP secretion (B) and TxA2 production (C). Therefore, αIIbβ3-mediated aggregation is required for maximum ATP secretion and TxA2 production in response to a low level of collagen. Data were obtained from 6 tests. Bars represent means ± SEM.

A low concentration of collagen-induced, αIIbβ3-mediated aggregation drives ADP secretion and TxA2 production.

Wild-type platelets were incubated with 1B5, a hamster monoclonal antimouse αIIbβ3 antibody (10 μg/mL), or control hamster IgG (10 μg/mL) for 10 minutes before stimulation with a low concentration (2.5 μg/mL) of collagen. 1B5 inhibits fibrinogen binding to αIIbβ3, thereby preventing aggregation (A). Inhibition of aggregation substantially diminished ATP secretion (B) and TxA2 production (C). Therefore, αIIbβ3-mediated aggregation is required for maximum ATP secretion and TxA2 production in response to a low level of collagen. Data were obtained from 6 tests. Bars represent means ± SEM.

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