Fig. 2.
Distribution of AML CFCs and LTC-ICs in different phases of the cell cycle as determined by Hst/PY staining.
AML cells were stained with Hst/PY and sorted into 3 different subpopulations based on their DNA and RNA content as described in Figure 1, following which they were then plated into CFC and LTC-IC assays. The percentage of total CFCs or LTC-derived colonies in each sorted subpopulation was calculated by multiplying the number of progenitors detected per 106 sorted cells from that population by the percentage of the total cell population represented by that subpopulation and then dividing that number by the total number of progenitors found in all 3 sorted subpopulations (× 100).
