Expression of Nur77 and PAI-1 in HUVECs induced with TNFα.

(A) Q-PCR analysis of Nur77 and PAI-1 mRNA expression. HUVECs were induced with TNFα for the indicated minutes; relative expression was normalized to β-2 microglobulin. The inset shows a Western blot of HUVECs stimulated for the indicated number of seconds with TNFα and stained using an antibody recognizing the N-terminus of Nur77 (representative experiment). (B) Immunocytochemistry. HUVECs were stimulated by TNFα for indicated periods and stained with antibodies recognizing Nur77 or PAI-1. (C) Q-PCR analysis of Nur77 and PAI-1 mRNA expression. HUVECs were induced with LPS and IL-1 for the indicated time periods (±SD of triplicates).