Northern blot analysis of

FIG1 expression in human tissues and B-cell lines. (A) Premade Nylon membrane (Clontech) loaded with 2μg poly A+ RNA per lane from spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leukocytes. (B) Total RNA (5 μg) from thymus, spleen, tonsil, reactive lymph nodes (LN1, LN2, LN3), resting tonsillar B cells (B cells NA), and IL-4+CD40L–activated tonsillar B cells (B cells A) was loaded on each lane. Ethidium bromide–stained 28S and 18S rRNA bands are shown for comparison (top). (C) Total RNA (5 μg) from human B-cell lines was loaded on each lane. Ethidium bromide-stained 28S and 18S rRNA bands are shown for comparison (top). Membranes were hybridized with the α-³²P–labeled human FIG1 RDA fragment and the Clontech membrane (A) was also hybridized with aβ-actin probe as control.