Western blot analysis for cyclin D1, p27Kip1, and other cell cycle–related proteins in lymphoid cell lines.
Each lane contains 30 μg protein extract from the following cell lines: lane 1, Granta 519; lane 2, NCEB-1; lane 3, SUDHL-4; lane 4, SUDHL-6; lane 5, Thomas; lane 6, Molt4; lane 7, KiJK; lane 8, SUDHL-10; lane 9, KMS12. Lanes 1 (Granta 519), 2 (NCEB-1), and 9 (KMS12) represent the t(11;14) translocated cell lines. Expression of cyclin D1 is limited to these 3 cell lines. These cell lines also show strong positivity for p27Kip1 although several of the non-t(11;14) translocated cell lines also show relatively high levels of p27Kip1. Note that the cell lines with positivity for cyclin D1 have the strongest positivity for p27Kip1. Cyclin E, cyclin A, CDK4, and CDK2 expression is present in all cell lines with no apparent correlation between any of these proteins and either cyclin D1 or p27Kip1.
