Western blot analysis for cyclin D1, p27Kip1, and other cell cycle–related proteins in primary MCL.
Each lane contains 30 μg protein extract. Lane G represents the Granta 519 cell line control; lanes 1-7 represent 7 cases of typical MCL. Lanes 8 and 12-14 represent 3 control cases of CLL. Lanes 8 and 14 correspond to the same CLL case. Lanes 9-11 represent 3 cases of blastic MCL. Note that all the MCL cases express cyclin D1 and the CLL cases are negative for cyclin D1. p27Kip1 is expressed in all instances; however, its expression is highest in the blastic variant of MCL (lanes 9-11), followed by the CLL cases (lanes 8 and 12-14). Note that the expression of cyclin A, cyclin E, and CDK2 is very low in the typical MCL cases (lanes 1-7), whereas the expression of these proteins in the blastic variant of MCL is comparable to that of the cell lines. Cyclin A was not performed in the blastic cases because there was not enough tissue.
