Fig. 6.
Fig. 6. Coimmunoprecipitation studies of p27Kip1with cyclin E, cyclin A, and cyclin D1 in t(11;14) cell lines. / (A) The cell lines Granta 519 (G) and NCEB-1 (N) were immunoprecipitated with agarose-conjugated anti-p27Kip1. Western blots were probed with either p27Kip1, cyclin E, cyclin A, or cyclin D1. In both cell lines, the predominant cyclin recovered from p27Kip1 immunoprecipitates is cyclin D1. (B) In the inverse assay, the cell lines were immunoprecipitated with agarose-conjugated anticyclin E and blotted with polyclonal p27Kip1 antibody. p27Kip1 was recovered in cyclin E immunoprecipitates from NCEB-1. (C) The cell lines were immunoprecipitated with agarose-conjugated cyclin A and blotted with p27Kip1. A faint band is observed in Granta 519 and in NCEB-1. C indicates positive control, 60 μg total protein; IP, immunoprecipitation, 300 μg total protein.

Coimmunoprecipitation studies of p27Kip1with cyclin E, cyclin A, and cyclin D1 in t(11;14) cell lines.

(A) The cell lines Granta 519 (G) and NCEB-1 (N) were immunoprecipitated with agarose-conjugated anti-p27Kip1. Western blots were probed with either p27Kip1, cyclin E, cyclin A, or cyclin D1. In both cell lines, the predominant cyclin recovered from p27Kip1 immunoprecipitates is cyclin D1. (B) In the inverse assay, the cell lines were immunoprecipitated with agarose-conjugated anticyclin E and blotted with polyclonal p27Kip1 antibody. p27Kip1 was recovered in cyclin E immunoprecipitates from NCEB-1. (C) The cell lines were immunoprecipitated with agarose-conjugated cyclin A and blotted with p27Kip1. A faint band is observed in Granta 519 and in NCEB-1. C indicates positive control, 60 μg total protein; IP, immunoprecipitation, 300 μg total protein.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal