Fig. 4.
![Fig. 4. No evidence of ex vivo CD20 shedding. / (A) Supernatant from cultured cells of patients with CLL showed no significant increase with time regardless of whether the cells were cultured without or with a shedding agent (phorbol 12-myristate 13-acetate [PMA]). In contrast, in HLA class I, levels increased significantly in the supernatant from cells cultured with PMA. (B) Similar levels of cCD20 were detected whether plasma samples were separated from cells immediately after collection (0) or after a prolonged period at room temperature (1, 2, 4, 8, 16, 24, 36, and 48 hours).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/7/10.1182_blood-2002-06-1639/3/h80734070004.jpeg?Expires=1735549046&Signature=VaQavBMKar5lKcpR0KNzzryIlBpBASSSgJaZPFl8brLQy5z6XxoPtZKm0trspHTp8xGSBTPfRY5zTx0km1kJ7PZqCs5ItImEskTfzYzsxVk9W6hE37mzjCu0cIOTte7uYwYPkyls64xooftqTk76e8MG~8-oIIv-UfnyHTcLx230CjZ~dVgxzKYEw40K2Wgl~w6h8LOfAjP68yfl5WbYlgiuf8kLihNfrhGqNRxffPZVAE1Ry-X8F9PV7eYF5tyOPP00CG3E5EeHQ81pEXdJ7jXWgb1ONv4dwAKPZ69PGCUgiP-vqoSU9Z0P0-GOV~vKe8~ZJ57vrm-7ci-QCrJbKA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
No evidence of ex vivo CD20 shedding.
(A) Supernatant from cultured cells of patients with CLL showed no significant increase with time regardless of whether the cells were cultured without or with a shedding agent (phorbol 12-myristate 13-acetate [PMA]). In contrast, in HLA class I, levels increased significantly in the supernatant from cells cultured with PMA. (B) Similar levels of cCD20 were detected whether plasma samples were separated from cells immediately after collection (0) or after a prolonged period at room temperature (1, 2, 4, 8, 16, 24, 36, and 48 hours).
