SgIGSF expression in various tissues of B6^tg/tg mice.

(A) Expression of the SgIGSF protein in testis, lung, spleen, and stomach tissues of B6^+/+ and B6^tg/tgmice. Lysates were prepared from the indicated tissues and were blotted with the anti-SgIGSF antibody. (B) N-linked glycosylation of SgIGSF proteins in B6^+/+ CMCs and testes. Lysates of B6^+/+ CMCs and testes were incubated at 37°C for 1 hour in the presence (+) or absence (−) of PNGase F and blotted with the anti-SgIGSF antibody. The molecular weight scale is shown to the right of the blots. The blots were probed again with the anti–α-tubulin antibody to indicate the total amount of proteins loaded per lane.