Expression of the SgIGSF protein in B6^tg/tg and B6^mi/miCMCs transfected with SgIGSF cDNA.

B6^tg/tg CMCs were infected with the empty retroviral vector (tg/tg CMC^Vector) or the vector containing either SgIGSF cDNA (tg/tgCMC^SgIGSF) or +-MITF cDNA (tg/tgCMC^MITF+/−). B6^mi/mi CMCs were infected with the vector containing the SgIGSF cDNA (mi/miCMC^SgIGSF). Lysates of the indicated cells were electrophoresed and blotted with the anti-SgIGSF and anti-MITF antibodies. After stripping, the blot was probed with the anti–α-tubulin antibody to indicate the total amount of proteins loaded per lane.