Fig. 6.
Fig. 6. Phenotypes of B6tg/tg CMCs transfected with SgIGSF or +-MITF. / (A) Phase contrast image of B6tg/tgCMCs transfected with SgIGSF cDNA. Aggregates of the CMCs were floating in the medium. Bar, 50 μm. (B) Aggregates composed of numerous alcian blue–positive cells. Cytospin samples of B6tg/tg CMCs transfected with SgIGSF cDNA were stained with alcian blue and nuclear fast red. Bar, 50 μm. (C,D) Immunocytochemical analysis of B6tg/tg CMCs transfected with either SgIGSF cDNA (C) or +-MITF cDNA (D). Cytospin preparations of either type of cells were fixed with methanol, reacted with the anti-SgIGSF antibody, and stained with FITC-labeled secondary antibody. Bar, 10 μm.

Phenotypes of B6tg/tg CMCs transfected with SgIGSF or +-MITF.

(A) Phase contrast image of B6tg/tgCMCs transfected with SgIGSF cDNA. Aggregates of the CMCs were floating in the medium. Bar, 50 μm. (B) Aggregates composed of numerous alcian blue–positive cells. Cytospin samples of B6tg/tg CMCs transfected with SgIGSF cDNA were stained with alcian blue and nuclear fast red. Bar, 50 μm. (C,D) Immunocytochemical analysis of B6tg/tg CMCs transfected with either SgIGSF cDNA (C) or +-MITF cDNA (D). Cytospin preparations of either type of cells were fixed with methanol, reacted with the anti-SgIGSF antibody, and stained with FITC-labeled secondary antibody. Bar, 10 μm.

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