Fig. 7.
Fig. 7. Growth kinetics of SgIGSF-transfected CMCs. / CMCs (2.5 × 105) were suspended in 2 mL fresh medium containing PWM-SCM and plated onto 35-mm culture dishes. On the indicated days after plating, total cell numbers were counted with a standard hemocytometer. Four types of CMCs were examined: B6mi/mi CMCs (▪), B6tg/tg CMCs (●), B6mi/mi CMCs transfected with SgIGSF cDNA (mi/mi CMCSgIGSF; ■), and B6tg/tg CMCs transfected with SgIGSF cDNA (tg/tg CMCSgIGSF; ○). Mean cell numbers of triplicate samples were plotted; bars indicate SE. *P < .01 by Student t test when compared with the values of intact B6mi/mi or B6tg/tg CMCs.

Growth kinetics of SgIGSF-transfected CMCs.

CMCs (2.5 × 105) were suspended in 2 mL fresh medium containing PWM-SCM and plated onto 35-mm culture dishes. On the indicated days after plating, total cell numbers were counted with a standard hemocytometer. Four types of CMCs were examined: B6mi/mi CMCs (▪), B6tg/tg CMCs (●), B6mi/mi CMCs transfected with SgIGSF cDNA (mi/mi CMCSgIGSF; ■), and B6tg/tg CMCs transfected with SgIGSF cDNA (tg/tg CMCSgIGSF; ○). Mean cell numbers of triplicate samples were plotted; bars indicate SE. *P < .01 by Student t test when compared with the values of intact B6mi/mi or B6tg/tg CMCs.

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