Total bleeding induced by TPA or plasmin.

Total bleeding induced by TPA (1 mg/kg) or plasmin (8 mg/kg) relative to the time during which agent was infused. Results are expressed as mean of 5 animals for either treatment group. The preinfusion ear puncture bleeding times were performed at −30, −10, and 0 minutes before starting TPA or plasmin. During the 55- to 60-minute infusions, ear punctures were induced at 10 and 30 minutes; postinfusion bleeding times were performed at 60, 70, 90, and 120 minutes. TPA (░) caused increased bleeding from the sites induced before or during infusion, most evident at the 10-minute site, whereas plasmin (▪) caused bleeding from midpoint of infusion and at all subsequent ear puncture sites. Results for bleeding at 180 minutes are not shown because experiments were terminated just after these observations.