Differential distribution of Felic and CIP4 during phagocytosis.

RAW264.7 macrophages were transfected with HA-Felic (A-B,E-F) or HA-CIP4 (C-D) and allowed to bind and initiate phagocytosis of IgG-opsonized SRBCs (A-D) or serum-opsonized zymosan (E-F). The cellular distribution of HA-Felic and HA-CIP4 was often cytosolic. HA-Felic was strongly recruited to phagocytic cups during phagocytosis of IgG-opsonized SRBCs (A-B, arrows) and serum-opsonized zymosan (E-F, arrows) and quickly dissociated from sealed phagosomes (A-B,E-F, asterisks). In contrast to Felic, CIP4 was weakly recruited to the sites of phagocytosis (C-D, arrows). Panels B, D, and F show the corresponding differential interference contrast images of panels A, C, and E, respectively. Original magnifications × 1000.