Combination of activated FLT3 and PML-RARα results in aggressive disseminated acute myeloid leukemias.

(A,C,E,G,I) Healthy FVB/N control. (B,D,F,H,J) Representative findings of leukemic-activated FLT3/PML-RARα mice. (A-B) Peripheral blood. Leukocytosis, including immature forms/blasts with indented nuclei, is present in leukemic mice. Comparisons of normal neutrophil with abnormal immature form/blast (insets). (C-D) Bone marrow smears. Leukemic marrow is filled with immature forms/blasts, many with numerous azurophilic granules. (E-F) Spleens. Leukemic spleen has red pulp effaced with leukemic cells; insets show red pulp at higher magnification. (G-H) Livers. Leukemic cells are present in periportal areas and infiltrate the sinusoids. (I-J) Kidneys. Leukemic cells infiltrate the kidney focally. (A-D) Wright-Giemsa stain. (E-J) Hematoxylin and eosin stain. Original magnification × 400 (A-D, G-J). Original magnification × 2400 (A-B, insets). Original magnification × 100 (E-F; insets, × 400).