rhHMGB1 stimulates the release of fibrinolytic regulators tPA and PAI-1 from HMEC-1 cells.

Incubation of HMEC-1 cells with rhHMGB1 (50 ng/mL) over a 48-hour period resulted in an increased secretion of (A) tPA (P = .005 at 48 hours compared with medium) and (B) PAI-1 (P = .0006 at 48 hours compared with medium). Both molecules were constitutively secreted by HMEC-1 cells. TNFα stimulation resulted in higher tPA and PAI-1 levels compared with HMGB1. Data represent mean ± SEM of 4 separate experiments.