Enhanced binding of Gp96-FITC or LPS-FITC by activated PMNs.

Unstimulated PMNs (○) or PMNs prestimulated with PMA (10 ng/mL) for 10 minutes at 37°C (●) were incubated with the indicated concentrations of gp96-FITC (A) or LPS-FITC (B) and analyzed by FACS. The increase in mean fluorescence was statistically significant for both Gp96-FITC (50 μg/mL) or LPS-FITC (30 μg/mL) binding in 5 independent experiments (n = 5; P < .05 orP < .001, respectively, by Student ttest).