AKT mediates CD40-induced MM cell migration in a PI3K-dependent manner.

(A) MM.1S cells were infected with Ad dnAKT or Ad myrAKT for 2 hours, and fresh medium was then added. Cell lysates from infected cells, with or without CD40 stimulation, were probed using an anti-HA mAb for Ad myrAKT and an anti-GFP Ab for Ad dnAKT. (B) MM.1S cell were transduced with indicated adenoviruses overnight and stimulated with G28.5 mAb for indicated time intervals. Total cell lysates were prepared and phosphorylation of AKT was detected. (C) Infected MM.1S cells were pretreated with or without LY294002 (30 μM) for 1 hour, and then seeded in the upper chamber in the transwell cluster plate. Transmigration assays were performed as described in “Materials and methods.”