Effects of Ad dnAKT and Ad myrAKT on CD40-induced IκBα degradation.

Adenoviruses expressing kinase-dead AKT (Ad dnAKT) (A) and constitutively active AKT (Ad myrAKT) (B) were transduced into MM.1S cells, followed by serum starvation for 2 hours. Serum-starved adenovirus-transduced cells were incubated with 2 μg/mL G28.5 anti-CD40 mAb, and collected at the indicated time points. Cell lysates were subjected to Western blotting using anti-IκBα, and with anti–α-tubulin mAb as a loading control.