CyA and FK block deletion of donor-reactive CD4⁺ PBLs following BMT with cobl.

The percentage of Vβ11⁺ and Vβ5⁺CD4⁺ PBLs was examined by 2-color FCM at multiple time points. Results are shown as mean + SD. Vβ8⁺ CD4⁺ cells were not deleted in any group (data not shown), indicating that deletion occurring with this protocol was specific. All mice were treated with 3-Gy TBI, BMT, and cobl. Standard-protocol groups (♦; n's = 5 and 6) did not receive any further treatment. (A) ■ denotes a control group, which received only BMT and rapa (deletion did not occur in this group). Other groups were treated additionally with CyA (⋄; n = 6) or rapa (▴; n = 6). (B) Groups received additionally MP (■; n = 6), FTY720 (▴; n = 6), MMF (⋄; n = 6), or FK (●; n = 6). P values were calculated for comparison with deletion from the standard-protocol group of the respective experiment.