Denaturing HPLC profiles from representative AML patient DNA.

(A) Denaturing HPLC profile from DNA containing wild-type codon 12/13 NRAS sequence only. (B) Denaturing HPLC profile from DNA containing wild-type plus mutant sequences. In this profile, wild-type homoduplexes are retained on the dHPLC column for longer (6.36 minutes) than mutant/wild-type heteroduplexes (5.97 minutes).