American Society of Hematology

Fig. 4.

$Fig. 4. Molecular analysis of patient 1's T-lymphocyte complexity. / (A) Patient 1's PBLs were separated into TcR Vβ14-positive and -negative fractions, cut with restriction enzymesSstI (double cutter) and EcoRI (single cutter). SstI digests demonstrate that the vector DNA has not been rearranged. The smear in the lanes cut withEcoRI indicates that there have been multiple LASN integrations. The first lane of panels A, C, and D contains molecular weight markers. (B) Spectratyping of TcR CDR3 of Vβ14 in PBMCs from a healthy control subject and patient 1. (C) Southern blot analysis of single cell clones. SstI andEcoRI digests. (D) BglII (single cutter) digests.$

Molecular analysis of patient 1's T-lymphocyte complexity.

(A) Patient 1's PBLs were separated into TcR Vβ14-positive and -negative fractions, cut with restriction enzymesSstI (double cutter) and EcoRI (single cutter). SstI digests demonstrate that the vector DNA has not been rearranged. The smear in the lanes cut withEcoRI indicates that there have been multiple LASN integrations. The first lane of panels A, C, and D contains molecular weight markers. (B) Spectratyping of TcR CDR3 of Vβ14 in PBMCs from a healthy control subject and patient 1. (C) Southern blot analysis of single cell clones. SstI andEcoRI digests. (D) BglII (single cutter) digests.

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