PECAM-1–dependent transmigration is chemokine/cytokine specific.
Confluent RHP and REN monolayers treated with 5 nm IL-8 (abluminal) or 100 nm LTB4 (abluminal) at the start of the TM assay or with 10 U/mL IL-1β (luminal) for 24 hours as indicated. Monolayers and neutrophils were treated with luminal anti–PECAM-1 (Houston) or anti–MHC-1 antibodies (100 μg/mL) as indicated. Similar results were obtained using BSA instead of anti–MHC-1 antibody (not shown). Transmigration rates are expressed as the proportion of PMNs migrating through the transwell filter compared with the total number of PMNs added at the start of the experiment. Data represent the mean ± SEM from a minimum of 3 transwells for each condition from 2 separate experiments. (*Significantly different from all other columns,P < .05.)
