Fluorescence-activated cell-sorter (FACS) analysis of donor/host contribution to hematopoietic lineages of reconstituted mice.

Retro-orbital blood from animals reconstituted with E13.5 liver cells from wild-type (WT/WT) and WT1 mutant (−/−) embryos was incubated with antibodies to Ly5.2 and Ly5.1 specific for the donor and the lethally irradiated host, respectively. Cells were fractionated by size and side scatter into the lymphocyte (LYMPH), granulocyte (GRAN), and monocyte (MONO) populations and were analyzed for the expression of Ly5.1 (PE) and Ly5.2 (FITC) antigens.