Fig. 3.
Hematopoietic colony-forming potential of fetal liver cells.
Dissociated E14.5 fetal liver cells were cultured in Methocult GF M3434 (StemCell Technologies) in triplicate at 1.5 × 104cells/mL at 37°C and 5% CO2. CFU-Es were counted after 2 days of culture, CFU-GMs after 10 days of culture, and CFU-GEMMs after 14 days of culture. (A-C) CFU-E, CFU-GM, and CFU-GEMM per 1.5 × 104 fetal liver cells, respectively. (D-F) CFU-E × 10−4, CFU-GM × 10−4, and CFU-GEMM × 10−3 per fetal liver, respectively. Fifteen KO/KO embryos, 20 KO/WT embryos, and 16 WT/WT embryos were used in these analyses. KO indicates knockout.
