Genomic organization and multiple amino acid alignments of IGnT and novel IGnT-homologous sequences.

(A) Two BAC clones containing novel IGnT-homologous sequences. 2 BAC clones, RP11-360O19 and RP11-421M1, are illustrated. Boxes represent exons. RP11-360O19 contains IGnT1-exon 1 and a sequence homologous to IGnT1-exon 1 (IGnT2-exon 1), and RP11-421M1 contains exons 2 and 3 of IGnT1 and another sequence homologous to IGnT1-exon 1 (IGnT3). Arrowheads above the exons indicate the positions of PCR primers designed in the present study. (B) Genomic structure of the human IGnT1, IGnT2, and IGnT3. These 3 genes are arranged as 3 exons contained within the sequence of NT_007291 from the chromosome 6 working-draft sequence segment. Nucleotides from 219 000 to 319 000 of NT_007291 are illustrated in this figure. NT numbers are assigned to and are guaranteed as the high-quality genome sequences by NCBI. A scale bar of 2 kb is presented at the right bottom corner. (C) Multiple amino acid sequence analysis of human IGnT1, IGnT2, and IGnT3. Identical residues are indicated by boxes. Putative transmembrane domains are underlined. The positions of conserved cysteines are indicated by asterisks, and the splicing sites are indicated by arrows.