![Fig. 3. Shear stress inhibits the gene expression of ICAM-1, VCAM-1, and E-selectin in ECs induced by coculture with SMCs. / ECs cultured alone (EC/φ) or cocultured with identical ECs (EC/EC) or SMCs (EC/SMC) were maintained in a static condition or exposed to a shear stress of 12 dyne/cm2 for 6 hours and their mRNA levels were determined by RT-PCR analysis, as described in “Materials and methods.” Amplification of cDNA was performed in parallel samples using human GAPDH primers. Data are presented for expression of ICAM-1 (A), VCAM-1 (B), and E-selectin (C) as a percentage change in band density normalized to GAPDH samples (ie, differences from densities in static control ECs cultured alone [Control EC/φ]), and are shown as mean ± SEM from 4 independent experiments. *P < .05 vs control EC/φ.#P < .05 vs control EC/SMC.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/7/10.1182_blood-2002-08-2560/3/h80734041003.jpeg?Expires=1735551321&Signature=gKqgSzo2QFxAXy0zig0lu1p-6B77pyDvZzaUoRdf18OpgoRCaX21KBIALFl1~v3OVB5Y4SMcILtkaPUkD1qnlvfJgXDlf-T2VSDAojK3wclQA-mLQzFXsUxNKKmLmTKUg3W2X~eCmjozz8cqmLe306-Atj3V~5SIk8RQcRR7s2gJ0KL6xhvfXA212dHnGHF0q-ZHMWJXI3hQ87Ye220f-Jxsk~theZtJbrky38~PHjCJVaJufC8xnP1BDgDkqP3s6ZPxkBeN4aMXtqaiAkQ6WhqarF97y1R4ZUZjuaAv5k8OHZG037Zypsej9eeBuLcAl~m8uHKEO1pRsi0ic5B6BA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Shear stress inhibits the gene expression of ICAM-1, VCAM-1, and E-selectin in ECs induced by coculture with SMCs.
ECs cultured alone (EC/φ) or cocultured with identical ECs (EC/EC) or SMCs (EC/SMC) were maintained in a static condition or exposed to a shear stress of 12 dyne/cm2 for 6 hours and their mRNA levels were determined by RT-PCR analysis, as described in “Materials and methods.” Amplification of cDNA was performed in parallel samples using human GAPDH primers. Data are presented for expression of ICAM-1 (A), VCAM-1 (B), and E-selectin (C) as a percentage change in band density normalized to GAPDH samples (ie, differences from densities in static control ECs cultured alone [Control EC/φ]), and are shown as mean ± SEM from 4 independent experiments. *P < .05 vs control EC/φ.#P < .05 vs control EC/SMC.
