NO modulates SMC-induced ICAM-1, VCAM-1, and E-selectin gene expression in cocultured ECs under the static condition.

(A) NO donor attenuates SMC-induced ICAM-1, VCAM-1, and E-selectin mRNA levels in cocultured ECs. ECs were pretreated with SNAP or DETA/NO for 30 minutes at a concentration of 100 μM and then cocultured with SMCs in the presence of the respective NO donors. Data represent duplicate experiments with similar results. (B) ECs cocultured with SMCs reduced their eNOS mRNA expression. ECs cultured alone (EC/φ) or cocultured with identical ECs (EC/EC) or SMCs (EC/SMC) were maintained in the static condition and their eNOS mRNA levels were determined by RT-PCR analysis, as described in “Materials and methods.” Amplification of cDNA was performed in parallel samples using human GAPDH primers. Data are presented as percentages of control EC/φ in band density normalized to GAPDH RNA levels, and are shown as mean ± SEM from 4 independent experiments. *P < .05 vs control EC/φ.