Effect of Dex on erythroid cell growth and maturation.
(A) Growth curve from minibulk HPC erythroid cultures supplemented or not supplemented with KL (100 ng/mL) and graded amounts of Dex (10−9 to 10−6 M). C indicates control. Total cell number (left) and percentage of mature erythroblasts (polychromatophilic 2 + orthochromatic erythroblasts) during erythroid differentiation (right) are shown. Mean ± SEM values from 5 separate experiments. Variance analysis was performed to evaluate the difference between KL and each Dex dose.P < .001 when comparing KL with Dex 10−7 or 10−8 M. The difference between KL and Dex 10−6 or 10−9 M is not significant. (B) Left panel, total cell number from minibulk erythroid cultures supplemented only with Dex; right panel, erythroid maturation, as evaluated in terms of mature erythroblasts on days 14 to 16 of culture. C indicates control. Mean ± SEM values from 4 separate experiments.P < .01 and P < .001 when comparing C with Dex 10−7 and 10−8 M, respectively.
