Figure 3.
![Figure 3. Relative activity of hepcidin derivatives. (A) Flow cytometry measuring degradation of ferroportin-GFP. (B) Ferritin ELISA measuring cellular iron retention. For both panels, the values were expressed as a fraction of hep25 activity. Peptides were added to cells for 24 hours at 1 μM concentration, except for hep3 and hep6, which were added at 4 μM. 25 = hep25; 24 to 20 = del1(D) to del1-5(DTHFP); 3 = hep3 (DTH); 6 = hep6 (DTHFPI); 26 = hep26(+A); dKT = del24-25(KT); AA1 = C7A/C13A; AA3 = C11A/C19A; AA4 = C13A/C14A; 1SS = [del9-12(FCCG), del19-22(CGMC), C13A/C14A]; zebra = zebra fish. The activities corresponding to the unmodified, naturally occurring active forms of hepcidin are shown as black bars.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/1/10.1182_blood-2005-05-2049/4/zh80010688580003.jpeg?Expires=1735527551&Signature=hXLNqcKnE-4HJ6nE6JHn9SLG~BAkZlmX9IrJTnvbE9jnkv2EpBNcYYPqWKeNThNEcNm9ji9RBKukCjszmTa6QLASvZLz2jzRoabDXzBTdfzmVAydjthLToYzM0Uwy~5SlL0PpLBS23IXSN-JAidpM5XlkrK-oTx4DavClfw~RlbeY8nXYYme8BP-cROIZHIk1MsemUxVU09rdu9-MqIG44NLc3kv025nDsfDUuR4pU4URgGrO69ARsYXzun5Q5k4dOwHF8bynSeLTIffDjwZQgsLAhqTaZGBF6s8jlDTQNCfbNgm0vF4kiL18huw7dA6i3SorXxS1LiAv7TPSqff6w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Relative activity of hepcidin derivatives. (A) Flow cytometry measuring degradation of ferroportin-GFP. (B) Ferritin ELISA measuring cellular iron retention. For both panels, the values were expressed as a fraction of hep25 activity. Peptides were added to cells for 24 hours at 1 μM concentration, except for hep3 and hep6, which were added at 4 μM. 25 = hep25; 24 to 20 = del1(D) to del1-5(DTHFP); 3 = hep3 (DTH); 6 = hep6 (DTHFPI); 26 = hep26(+A); dKT = del24-25(KT); AA1 = C7A/C13A; AA3 = C11A/C19A; AA4 = C13A/C14A; 1SS = [del9-12(FCCG), del19-22(CGMC), C13A/C14A]; zebra = zebra fish. The activities corresponding to the unmodified, naturally occurring active forms of hepcidin are shown as black bars.
