Antiproliferative effects of mTOR blockade are equally effective under BM-like biomechanical conditions. (A) HUVECs, BAECs, and BM-ECs were cultured for 24 hours in a 12-channel perfusion bioreactor in cytokine media/EGM2 and subjected to venouslike (2 dyn/cm², steady flow), arterial-like (18 dyn/cm², 1 Hz pulsatile flow), or BM-like flow (0.5 dyn/cm², steady flow). As control, cells subjected to no-flow conditions were also tested. The results are presented as the ratio between the number of seeded cells and the final number of collected cells (cell proliferation expressed as fold increase). Error bars indicate SD. ^*Significance level of P < .005 compared with arterial-like flow. ^▴Significance level of P < .001 compared with venouslike flow or no flow. (B) BM-ECs were cultured for 3 days in the perfusion bioreactor under BM-like flow conditions in cytokine media/EGM2 alone (Control) or with CCI-779 (10 or 100 nM). Data represent the percentage of proliferation inhibition of drug-treated versus untreated BM-ECs, and error bars indicate SD. The results were obtained with BM-ECs from 3 different donors. ^*Significance level of P < .005 using a 2-tailed paired t test.