LSK⁺ cells and LSK^– cells show differential expression of motility-associated proteins. (A) Histogram showing the relative protein (▦) and mRNA (▪) expression ratios of cell-motility associated proteins between LSK⁺ and LSK^– cells. For gene name definitions, see Table 3. All proteins were significantly different (P < .05) between the 2 cell types. (B) Transwell migration assay to compare the motile responses of LSK⁺ and LSK^– cells. Basal motility and chemokinetic responses to LPA and S1P were assessed in the presence or absence of the chemotactic factor SDF-1.15 Results shown are the mean values for the numbers of cells that migrated to the bottom chamber, expressed as a percentage of total cells present (n = 3). Significantly different responses were seen between LSK⁺ cells and LSK^– cells for all conditions (P < .05, n = 3).