FCM analysis of N-Met-MCK-2 recruited Mac-1⁺/Gr-1^int myeloid cells. (A) N-Met-MCK-2-induced swelling. BALB/c mice were injected into FPs 4 times with 200-ng doses of N-Met-MCK-2 (♦) or PBS carrier (⋄) using groups of mice as indicated by arrowheads below the graph. Standard deviation of mean swelling is indicated by error bars. (B) FCM analysis of cells recovered from FPs prepared from 2 independent experiments using groups of 4 (experiment 1) or 5 (experiment 2) BALB/c mice at 3 hours after the fourth N-Met-MCK-2 injection. FCM analysis and layout is described in the legend to Figure 2.