FCM analysis of Mac-1⁺/Gr-1^int cells for CD31, Sca-1, and c-kit. FCM analysis of the FP infiltrates from groups of 10 infected BALB/c mice at 48 hours after inoculation with RQ461 (inoculum of 1 × 10⁶ PFUs) or mice at 4 hours after the fourth N-Met-MCK-2 injection. (A) Histograms of Mac-1⁺/Gr-1^int (solid box) or Mac-1⁺/Gr-1^high population (dotted box) FP cells from RQ461-infected mice, showing expression levels of CD31 (PECAM-1), Sca-1 (Ly-6A/E), and c-kit (CD117). (B) Histograms of Mac-1⁺/Gr-1^int (solid box) or Mac-1⁺/Gr-1^high (dotted box) FP cells from N-Met-MCK2-treated mice showing expression levels of CD31 (PECAM-1). Background staining with an isotype antibody control is indicated as a dashed line in each histogram.