MCMV replication and dissemination in MCP-1^-/- CCR2^-/- mice. (A) Time-course analysis of FP swelling in 5 BALB/c (▪) and 5 MCP-1^-/- CCR2^-/- (▦) mice following inoculation with 1 × 10⁶ PFUs of mck-expressing RM427⁺.¹⁴  The mean and standard deviation (error bars) are indicated. (B) Viremia levels determined by ICA on groups of 3 mice at day 5 after inoculation (intraperitoneal route) with 10⁵ PFUs of wild-type RQ461 in BALB/c and MCP-1^-/- CCR2^-/- mice. Mean (vertical line) and standard deviation (error bars) are indicated, along with data points from individual mice. (C) Titers of virus in spleen, lungs, and liver of RQ461 in BALB/c and MCP-1^-/- CCR2^-/- mice (same infection as panel A). (D) FCM analysis of Mac-1⁺/Gr-1^int myeloid cells recovered from FP of MCP-1^-/- CCR2^-/- mice. FP leukocytes were prepared from groups of 3 mice at day 5 after inoculation with mck-expressing RM427⁺ or mck-mutant RM461 lacZ-tagged viruses (inoculum of 1 × 10⁶ PFUs). FCM analysis and layout is described in the legend to Figure 2. (E) Day 14 virus titers in SGs following inoculation (FP route) with 10⁷ PFUs of RQ461 (▦) or RM461 (□).