Figure 4.
Figure 4. Early developmental expression of the Flk1+/lacZ neo-out allele. Expression of lacZ in 6.5 to 8.5 dpc Flk1+/lacZ neo-out embryos (A-O) and Flk1+/lacZ del Sw/B neo-out embryos (P-Q). (A) Mid-streak stage. (B) Transition between mid-streak stage and late-streak stage. Note that Flk1+ cells are located in embryonic lateral mesoderm. (C) Transition between late-streak stage and no-bud (OB) stage. Note that Flk1+ cells are seen in extraembryonic mesoderm layer as well as embryonic mesoderm. (D) Early bud (EB) stage. Note that Flk1+ cells are seen in broad region in extraembryonic and intraembryonic mesoderm. Panels E-H show sections at the levels marked. (E) A proximal tip of Flk1+ cells located at extraembryonic mesoderm. (F) A section through the extraembryonic region. (G) A section at the embryonic region most proximal to extraembryonic region. Note that most of mesodermal cells except for primitive streak (PS) region are Flk1+. (H) A section at a distal embryonic region. (I) Lateral view of Flk1+/lacZ neo-out embryo at late head-fold (LHF) stage after 4 hours of X-gal staining. (J) A sagittal section of panel I. Note that lacZ signal is seen in most of the extraembryonic mesoderm including blood island cells, mesothelial, amniotic, chorionic and allantoic mesoderm, and also in some embryonic mesoderm. Bl indicates blood island; ang, angioblast; AM, amniotic mesoderm; al, allantois; em, embryonic mesoderm. (K) A transverse section at heart level after 4 hours of X-gal staining. Note that lacZ is seen in endothelial cells of dorsal aorta, splanchnic mesoderm, endocardium, and some cells of the myocardium. (L) Higher magnification of the heart in panel K. (M) A transverse section at heart level after 24 hours of X-gal staining. Note that many myocardial cells are Flk1+. (N) A transverse section at somite level. Activity of lacZ is seen in cells of the dorsal part of the somite. (O) Higher magnification of Flk1+ cells in panel N. (P) Lateral view of Flk1+/lacZ del Sw/B neo-out embryo at LHF stage after 4 hours of X-gal staining. Note that there is no significant difference between panels I and P. (Q) A sagittal section of panel P. Note that there is no significant expressional change between panels J and Q.

Early developmental expression of the Flk1+/lacZ neo-out allele. Expression of lacZ in 6.5 to 8.5 dpc Flk1+/lacZ neo-out embryos (A-O) and Flk1+/lacZ del Sw/B neo-out embryos (P-Q). (A) Mid-streak stage. (B) Transition between mid-streak stage and late-streak stage. Note that Flk1+ cells are located in embryonic lateral mesoderm. (C) Transition between late-streak stage and no-bud (OB) stage. Note that Flk1+ cells are seen in extraembryonic mesoderm layer as well as embryonic mesoderm. (D) Early bud (EB) stage. Note that Flk1+ cells are seen in broad region in extraembryonic and intraembryonic mesoderm. Panels E-H show sections at the levels marked. (E) A proximal tip of Flk1+ cells located at extraembryonic mesoderm. (F) A section through the extraembryonic region. (G) A section at the embryonic region most proximal to extraembryonic region. Note that most of mesodermal cells except for primitive streak (PS) region are Flk1+. (H) A section at a distal embryonic region. (I) Lateral view of Flk1+/lacZ neo-out embryo at late head-fold (LHF) stage after 4 hours of X-gal staining. (J) A sagittal section of panel I. Note that lacZ signal is seen in most of the extraembryonic mesoderm including blood island cells, mesothelial, amniotic, chorionic and allantoic mesoderm, and also in some embryonic mesoderm. Bl indicates blood island; ang, angioblast; AM, amniotic mesoderm; al, allantois; em, embryonic mesoderm. (K) A transverse section at heart level after 4 hours of X-gal staining. Note that lacZ is seen in endothelial cells of dorsal aorta, splanchnic mesoderm, endocardium, and some cells of the myocardium. (L) Higher magnification of the heart in panel K. (M) A transverse section at heart level after 24 hours of X-gal staining. Note that many myocardial cells are Flk1+. (N) A transverse section at somite level. Activity of lacZ is seen in cells of the dorsal part of the somite. (O) Higher magnification of Flk1+ cells in panel N. (P) Lateral view of Flk1+/lacZ del Sw/B neo-out embryo at LHF stage after 4 hours of X-gal staining. Note that there is no significant difference between panels I and P. (Q) A sagittal section of panel P. Note that there is no significant expressional change between panels J and Q.

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