PTX3 regulates the production of cytokines by maturing DCs. The ability of DCs to secrete TNF-α (y-axis; A), IL-10 (y-axis; B), and TGF-β (y-axis; C) was assessed in the presence of PTX3 (50 μg/mL), LPS (10 ng/mL), apoptotic cells (AC), or a combination of these stimuli. The effect of CRP (50 μg/mL) on TNF-α secretion (y-axis) by stimulated DCs in the presence or in the absence of apoptotic cells is also reported in panel D. ^*P < .05, ^**P < .01; significantly different from control. Results representative of three independent experiments are depicted as mean ± SD of duplicate samples.