Figure 5.
Figure 5. PTX3 interferes with the cross-presentation of MelanA/MART1 to CD8+ T cells upon processing of apoptotic cells. MelanA/MART1-specific, HLA-A2-restricted CD8+ T cells secrete significant amounts of IFN-γ (x-axis; pg/mL) when challenged with T2 cells in the presence of the specific synthetic epitope (residues 27-35) or when challenged with HLA-A2+ (but not with HLA-A2- melanoma cell lines expressing the native antigen). Moreover, they secreted amounts of IFN-γ significantly higher than the background when challenged with DCs that phagocytosed and processed HLA-A2- apoptotic cells expressing MelanA/MART1; T-cell activation abated in the presence of PTX3 (50 μM). **P < .01, significantly different from control. Results are depicted as mean ± SD of samples analyzed in triplicate.

PTX3 interferes with the cross-presentation of MelanA/MART1 to CD8+ T cells upon processing of apoptotic cells. MelanA/MART1-specific, HLA-A2-restricted CD8+ T cells secrete significant amounts of IFN-γ (x-axis; pg/mL) when challenged with T2 cells in the presence of the specific synthetic epitope (residues 27-35) or when challenged with HLA-A2+ (but not with HLA-A2- melanoma cell lines expressing the native antigen). Moreover, they secreted amounts of IFN-γ significantly higher than the background when challenged with DCs that phagocytosed and processed HLA-A2- apoptotic cells expressing MelanA/MART1; T-cell activation abated in the presence of PTX3 (50 μM). **P < .01, significantly different from control. Results are depicted as mean ± SD of samples analyzed in triplicate.

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