American Society of Hematology

Figure 1.

$Figure 1. Coadministration of 2-ME and the HDACIs, NaB and SAHA, induces apoptosis in U937 cells in dose- and time-dependent manners. (A) U937 cells were treated with 0.5 μM 2-ME in the presence or absence of the designated concentration of NaB for 24 hours. (B) U937 cells were treated with 0.5 μM 2-ME in the presence or absence of the designated concentration of SAHA for 24 hours. (C) U937 cells were treated with either NaB (1 mM) or SAHA (1.5 μM) in the presence or absence of the designated concentration of 2-ME for 24 hours. (D) U937 cells were exposed to 0.5 μM 2-ME combined with either NaB (1 mM) or SAHA (1.5 μM) for the indicated time interval. After treatment as described, cells were stained with annexin V-FITC/PI, and apoptosis was determined using flow cytometry as described in “Materials and methods.” U937 cells were exposed to varying concentrations of 2-ME and NaB (E) or SAHA (F) at a fixed ratio (1:2 or 1:3, respectively) for 24 hours, after which the extent of apoptosis was determined by annexin V/PI staining and flow cytometry. CI values for each fraction affected were determined using commercially available software (Calcusyn, Biosoft). CI values less than 1.0 correspond to synergistic interactions.$

Coadministration of 2-ME and the HDACIs, NaB and SAHA, induces apoptosis in U937 cells in dose- and time-dependent manners. (A) U937 cells were treated with 0.5 μM 2-ME in the presence or absence of the designated concentration of NaB for 24 hours. (B) U937 cells were treated with 0.5 μM 2-ME in the presence or absence of the designated concentration of SAHA for 24 hours. (C) U937 cells were treated with either NaB (1 mM) or SAHA (1.5 μM) in the presence or absence of the designated concentration of 2-ME for 24 hours. (D) U937 cells were exposed to 0.5 μM 2-ME combined with either NaB (1 mM) or SAHA (1.5 μM) for the indicated time interval. After treatment as described, cells were stained with annexin V-FITC/PI, and apoptosis was determined using flow cytometry as described in “Materials and methods.” U937 cells were exposed to varying concentrations of 2-ME and NaB (E) or SAHA (F) at a fixed ratio (1:2 or 1:3, respectively) for 24 hours, after which the extent of apoptosis was determined by annexin V/PI staining and flow cytometry. CI values for each fraction affected were determined using commercially available software (Calcusyn, Biosoft). CI values less than 1.0 correspond to synergistic interactions.

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