American Society of Hematology

Figure 7.

$Figure 7. The effect of sodium arsenite on the levels of IκB-α, NF-κB, and survivin in nuclear and cytosolic fraction from MT2 and S1T cells. (A) MT2 cells were treated with sodium arsenite at the indicated concentrations; nuclear and cytosolic fractions were prepared at the indicated time. Nuclear protein (50 μg) was subjected to Western blotting using the antibody against p50, p65, or survivin. Cytoplasmic fraction (100 μg of protein) was subjected to Western blotting using the specific antibody against IκB-α or survivin. α-tubulin expression was used as a loading control. NE indicates nuclear extract; CE, cytoplasmic extract. *Nonspecific band. (B) p50 and p65 in the nucleus and cytoplasmic survivin in S1T cells treated with sodium arsenite in the indicated concentration, and time was measured by Western blot as in panel A.$

The effect of sodium arsenite on the levels of IκB-α, NF-κB, and survivin in nuclear and cytosolic fraction from MT2 and S1T cells. (A) MT2 cells were treated with sodium arsenite at the indicated concentrations; nuclear and cytosolic fractions were prepared at the indicated time. Nuclear protein (50 μg) was subjected to Western blotting using the antibody against p50, p65, or survivin. Cytoplasmic fraction (100 μg of protein) was subjected to Western blotting using the specific antibody against IκB-α or survivin. α-tubulin expression was used as a loading control. NE indicates nuclear extract; CE, cytoplasmic extract. *Nonspecific band. (B) p50 and p65 in the nucleus and cytoplasmic survivin in S1T cells treated with sodium arsenite in the indicated concentration, and time was measured by Western blot as in panel A.

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